Abstract：The extraction parameters for oil extraction from Lycium barbarum seed including extraction pressure,temperature and time were optimized using an orthogonal test design. The optimum conditions forsupercritical CO2 extraction were as follows: extraction pressure, 30 MPa; extraction temperature, 45 _C;dynamic extraction time, 60 min; CO2 flow, 25 kg/h. The oil yield under the conditions proposed was19.28 g/100 g. The effect of cell wall breakage pretreatment was investigated by supercritical CO2 rapid depressurization, and results indicated this pretreatment could result in a rapid and efficient extraction.A sensitive fluorescent reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl 4-methylbenzenesulfonate (BCETS) was utilized as pre-column labeling regent to determine fatty acids (FA) from Lycium barbarum seed oils obtained by different extraction methods. The main FA were: C18:2, C18:1, C16, C20:6, C18:3, and C20.The oil from L. barbarum seed exhibited excellent antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl（DPPH）radical scavenging assay and b-carotene bleaching test, and its antioxidant activity compared well with the references ascorbic acid and a- tocopherol. （IF=2.292，全文链接见附件）