Jing Jia（贾静）, Tao Chen（陈涛）, Ping Wang（王萍）,Guichen Chen（陈桂琛）,Jinmao You（尤进茂）,Yongjun Liu（刘永军）,and Yulin Li*（李玉林）.Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography, Phytochemical Analysis. 2012 ,23:332-336 （全文链接见附件）

Abstract：

Introduction – Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities,including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, antiinflammatory,anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendlymethods for isolating and purifying xanthones from S. mussotii are not currently available.

Objective – To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC).

Methodology – A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL/min, a rotation speed of 800 rpm and a temperature of 25℃.

Results – Using the described method, 8mg of methylswertianin, 21mg of swerchirin and 11mg of decussatin with purities of over 98% could be isolated from a 150mg crude sample. They were identified by 1H-NMR and 13 C-NMR analysis.

Conclusion – Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC.