陈光,JunLi,CuihuaSong,索有瑞,尤进茂*.A sensitive and efficient method for simultaneous trace detection and identification of triterpene acids and its application to pharmacokinetic study, Talanta.2012,98:101-111.IF=3.794

Abstract:A sensitive and efficient method for simultaneous trace detection of seven triterpene acids was developed and validated for analysis of rat plasma samples.The required micro-sampling of only 20mL blood reduced the difficulty in blood collection and the injury to animal.The whole pretreatment procedure was more Conveniently finished within 26min through the application of the semi-automated derivatizati on Extraction method to biological samples.Seven analytes were rapidly separated within 30minon reversed-phase Akasil-C18 column and quantified by fluorescence detector.Online ion trap MS with atmospheric pressure chemical ionization(APCI) source was used for further identification.Thenovel application of artificial neural network (ANN) combined with genetic algorithm (GA) to optimization of derivatization was performed and compared with the classical response surface methodology (RSM). Optimal derivatization condition was validated by multi-criteria and nonparametric tests and used Successfully to achieve the rather high sensitivity (limit of detection:0.67–1.08ng/mL).The limit of Reactant concentration (LORC) special for derivatization was studied and the lower values (2.53–4.03ng/mL) Ensured the traced etection.Results of validation demonstrated the advantages for pharmacokinetic study, Such as high ersensitivity,better accuracy,easier pretreatment and shorter run-time.Pharmacokinetic study of triterpene acids after oral administration of Salvia miltiorrhiza extract to mice was conducted for the first time.The present method provided more sensitive and  efficient alternative for the medical detection of bioactive constituents from herbal extract in the biological liquid.