陈晨, 陈涛, 刘永玲, 邹登朗, 尤进茂, 李玉林*, Rapid Screening, Identification,Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch, Separation Science and Technology, 2015, 50:604-610.

　　该研究首先利用HPLC-MS技术检测出川西獐牙菜中龙胆苦苷、芒果苷、獐牙菜苷和异荭草苷四种目标化合物主要存在于正丁醇萃取部位中，然后利用HSCCC和Sephadex LH-20技术成功分离得到了四种高纯度目标化合物。所用方法简单、高效、不存在样品死吸附问题。

　　Abstract：Gentiopicroside, mangiferin, sweroside, and isoorientin, the bioactive constituents of Swertia mussotii Franch, have various pharmacological effects, and are used in particular for treating liver disorders. However, efficient methods for their separation are not currently available. In this study, these bioactive compounds were detected in S. mussotii extracts using high-performance liquid chromatography coupled with mass spectrometry, and separated using a combination of high-speed counter-current chromatography and Sephadex LH-20 column chromatography. Their structures were determined by using 1H and 13C nuclear magnetic resonance spectroscopies. The results show that this method is effective for the separation and purification of bioactive compounds from S. mussotii.